BIOCHEMISTRY
Jaffe’s Method for the Estimation of Creatinine
B. Estimation of Creatinine Blank Standard Test Picric acid 1 ml 1 ml 1 ml Sodium hydroxide 1 ml 1 ml 1 ml Distilled water 3 ml – – Standard filtrate – 1 ml – […]
BIOCHEMISTRY
BIOCHEMISTRY
Colorimeter : Principle, Instrumentation and Uses
Colorimeter is a form of photometer which deals with the measurement of light transmitting power of a colored solution in order to determine the concentration of light absorbing substances present within it. It was invented […]
BIOCHEMISTRY
GOD-POD Method For The Estimation Of Blood Glucose
Glucose is the major carbohydrate present in the blood. It serves as a principal fuel for all the body tissues including the brain. It undergoes a series of chemical reactions to produce energy. Accurate and […]
BIOCHEMISTRY
Folin-Wu Method For Estimation of Blood Glucose
Folin-Wu method is one of the oldest methods for the estimation of blood sugar. However, it is almost obsolete for now but is in use in countries where enzyme preparations are not easy to obtain. […]
BIOCHEMISTRY
How to Convert ‘g’ to RPM and Vice Versa in a Centrifuge?
Centrifuagtion is a technique that helps to separate mixtures by applying centrifugal force. A centrifuge machine works by using a principle of sedimentation. Under the influence of gravitational force, substances separate according to their density. […]
BIOCHEMISTRY
Diacetyl Monoxime (DAM) Method for Estimation of Urea
Urea is a waste product formed in liver following the breakdown of proteins. It passes into the blood, is filtered out of the kidneys and excreted in urine. Thus determination of blood urea is the […]
BIOCHEMISTRY
O-Toluidine Method for Estimation of Blood Glucose
The O-toluidine method is an older method of blood glucose estimation. This method is no longer used today because O-toluidine is believed to be a carcinogen and is replaced by enzymatic methods. This method is […]
BIOCHEMISTRY
Sulphosalicylic Acid Test for Proteinuria: Principle and Procedure
Most plasma proteins are too large to pass through the glomeruli of the kidney. The small amount of protein which does filter through is normally reabsorbed back into the blood by the kidney tubules. Only […]
BIOCHEMISTRY
Heat and Acetic Acid Test for Proteinuria: Principle and Procedure
Most plasma proteins are too large to pass through the glomeruli of the kidney. The small amount of protein which does filter through is normally reabsorbed back into the blood by the kidney tubules. Only […]
BIOCHEMISTRY
Gerhardt’s Test For Ketone Bodies: Principle, Procedure and Interpretation
The term ketone bodies refers to three intermediate products of fat metabolism, namely acetone (2%), acetoacetic acid (20%) and beta-hydroxybutyrate (78%). Under normal conditions, metabolized fats are completely broken down to water and carbon dioxide, […]