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Sudan-Black-B-Stain
CYTOPATHOLOGY

Sudan Black B Stain : Purpose, Principle, Procedure and Interpretation

November 18, 2018 Dhurba Giri 1

Sudan Black B (SBB) is a fat soluble dye which has very high affinity for neutral fats and lipids. SBB staining is useful for for the differentiation of Acute myeloid leukemia (AML) from Acute lymphoid […]

Coagulase-test
BACTERIOLOGY

Coagulase Test : Types, Principle, Procedure, Interpretation and Examples

November 7, 2018 Dhurba Giri 0

Coagulase is an enzyme that works in conjugation with normal plasma components to convert fibrinogen to finbrin and causes clotting of plasma. This enzyme is a good indicator of pathogenic potential of Staphylococcus aureus. As […]

Differences-between-e-histolytica-and-e-coli
MICROBIOLOGY

Differences Between Entamoeba histolytica and Entamoeba coli

November 6, 2018 Dhurba Giri 6

Genus Entamoeba includes a group of amoebic parasites. Among them Entamoeba histolytica is an important human pathogen causing amoebic dysentery as well as hepatic amoebiasis. Entamoeba coli is a commensal of large intestine, but doesn’t […]

Myeloperoxidase-stain
CYTOPATHOLOGY

Myeloperoxidase (MPO) Stain : Purpose, Principle, Procedure and Interpretation

November 6, 2018 Dhurba Giri 5

Myeloperoxidase is a lysosomal enzyme present in the primary azurophilic granules of neutrophils, eosinophils and to a certain extent, monocytes. Lymphocytes do not contain myeloperoxidase. Purpose of Myeloperoxidase stain Myeloperoxidase (MPO) stain is useful for […]

Reticulocyte-Count
HEMATOLOGY

Reticulocyte Count : Principle, Procedure, Calculations and Clinical Significances

November 6, 2018 Dhurba Giri 7

Reticulocytes are immature red blood cells (RBCs) with considerable quantities of ribosomal and mitochondrial RNA. Normally, there are a small number of reticulocytes in the peripheral blood which remain for 24-48 hours during maturation. Reticulocyte […]

Hematoxylin and Eosin Staining
HISTOPATHOLOGY

Hematoxylin and Eosin (H&E) Staining : Principle, Procedure and Interpretation

November 5, 2018 Dhurba Giri 3

Hematoxylin and Eosin (H&E) staining is the most widely used staining technique in histopathology. As its name suggests, H&E stain makes use of a combination of two dyes, namely hematoxylin and eosin. This combination deferentially […]

Rothera's test for ketone bodies
BIOCHEMISTRY

Rothera’s Test for Ketone Bodies: Principle, Procedure and Clinical Significances

November 5, 2018 Dhurba Giri 5

The term ketone bodies refers to three intermediate products of fat metabolism, namely acetone (2%), acetoacetic acid (20%) and beta-hydroxybutyrate (78%). Under normal conditions, metabolized fats are completely broken down to water and carbon dioxide, […]

Urine-pregnancy-test
IMMUNOLOGY

Urine Pregnancy Test (UPT): Principle, Procedure, Interpretation and Limitations

December 25, 2017 Dhurba Giri 6

There are two types of tests for pregnancy testing: one uses blood sample and other uses urine. Both tests detect the presence of beta subunit of a hormone called human chorionic gonadotropin (hCG). The Urine […]

Reverse-blood-grouping
HEMATOLOGY

Reverse Blood Grouping: Principle, Procedure, Interpretation and Limitations

December 21, 2017 Dhurba Giri 16

Several blood grouping systems have been described in humans. Among these, ABO blood grouping system is the most important system for screening, transfusion and transplantation practice today. ABO blood grouping system divides blood into 4 […]

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