Colorimeter is a form of photometer which deals with the measurement of light transmitting power of a colored solution in order to determine the concentration of light absorbing substances present within it. It was invented […]
Glucose is the major carbohydrate present in the blood. It serves as a principal fuel for all the body tissues including the brain. It undergoes a series of chemical reactions to produce energy. Accurate and […]
Folin-Wu method is one of the oldest methods for the estimation of blood sugar. However, it is almost obsolete for now but is in use in countries where enzyme preparations are not easy to obtain. […]
Centrifuagtion is a technique that helps to separate mixtures by applying centrifugal force. A centrifuge machine works by using a principle of sedimentation. Under the influence of gravitational force, substances separate according to their density. […]
Urea is a waste product formed in liver following the breakdown of proteins. It passes into the blood, is filtered out of the kidneys and excreted in urine. Thus determination of blood urea is the […]
The O-toluidine method is an older method of blood glucose estimation. This method is no longer used today because O-toluidine is believed to be a carcinogen and is replaced by enzymatic methods. This method is […]
Most plasma proteins are too large to pass through the glomeruli of the kidney. The small amount of protein which does filter through is normally reabsorbed back into the blood by the kidney tubules. Only […]
Most plasma proteins are too large to pass through the glomeruli of the kidney. The small amount of protein which does filter through is normally reabsorbed back into the blood by the kidney tubules. Only […]
The term ketone bodies refers to three intermediate products of fat metabolism, namely acetone (2%), acetoacetic acid (20%) and beta-hydroxybutyrate (78%). Under normal conditions, metabolized fats are completely broken down to water and carbon dioxide, […]
Glycolysis is the major pathway for the utilization of glucose in the body. Glycolysis is defined as the sequence of reactions for the breakdown of Glucose (6-carbon molecule) to two molecules of pyruvic acid (3-carbon […]
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