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Gerhardts-Test
BIOCHEMISTRY

Gerhardt’s Test For Ketone Bodies: Principle, Procedure and Interpretation

December 12, 2020 Dhurba Giri 1

The term ketone bodies refers to three intermediate products of fat metabolism, namely acetone (2%), acetoacetic acid (20%) and beta-hydroxybutyrate (78%). Under normal conditions, metabolized fats are completely broken down to water and carbon dioxide, […]

laboratory-diagnosis-scrub-typhus
BACTERIOLOGY

Laboratory Diagnosis of Scrub Typhus

October 21, 2020 Dhurba Giri 0

Scrub typhus is an acute, febrile, infectious disease that is caused by bacteria called Orientia tsutsugamushi. It is a zoonotic disease (an animal disease that can spread to humans) primarily affecting rodents, rabbits and marsupials. […]

COVID-19-RT-PCR
MICROBIOLOGY

How COVID-19 RT-PCR Test Works? Principle and Protocol

September 20, 2020 Dhurba Giri 0

The coronavirus disease 2019 (COVID-19), is an illness caused by the most recently discovered coronavirus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It is an enveloped, positive sense, single-stranded ribonucleic acid (RNA) virus with […]

Wright's-Stain
CYTOPATHOLOGY

Wright’s Stain : Preparation, Principle, Procedure and Results

July 25, 2019 Dhurba Giri 4

Wright’s stain is a type of Romanowsky stain, which is commonly used in hematology laboratory for the routine staining of peripheral blood smears. It is also used for staining bone marrow aspirates, urine samples and […]

Megaloblastic-anemia
HEMATOLOGY

Laboratory Diagnosis of Megaloblastic Anemia (MBA)

July 21, 2019 Dhurba Giri 1

Anemia is a condition of decrease in number of circulating red blood cells (and hence hemoglobin) below a normal range for age and sex of the individual, resulting in decreased oxygen supply to tissues. Megaloblastic […]

Major-Cross-Matching
HEMATOLOGY

Cross Matching : Types, Principle, Procedure and Interpretation

July 2, 2019 Dhurba Giri 8

Cross matching is a procedure performed prior to transfusion of blood or blood products to detect any serological incompatibilities in the blood of donor and recipient. Before a donor’s blood is transfused into a recipient, […]

URINALYSIS

Hyaline Casts in Urine : Morphology And Clinical Significance

June 29, 2019 Dhurba Giri 2

Urinary casts are tiny cylindrical, tube-shaped particles that can be found during the microscopic examination of urine. Casts are the only elements found in the urinary sediment that are unique to the kidney. Tamm-Horsfall mucoprotein […]

Mantoux-Test
BACTERIOLOGY

Mantoux Test : Principle, Procedure, Interpretation and Limitations

June 24, 2019 Dhurba Giri 1

The Mantoux test is a qualitative, skin test to screen in vivo sensitization by Mycobacterium tuberculosis either due to active infection or past infection. It is also used to check the prophylaxis and efficacy of […]

Eggs-of-Ascaris-Lumbricoides
MICROBIOLOGY

Ascaris lumbricoides (Roundworm) Eggs: Morphology, Characteristics and Identification

March 26, 2019 Dhurba Giri 0

The Roundworm, Ascaris lumbricoides is the largest nematode parasite in human intestine. Disease caused by A. lumbricoides is called Ascariasis. Laboratory diagnosis of ascariasis can be made by demonstration of parasite (eggs, larvae or adult […]

Glycolysis
BIOCHEMISTRY

Glycolysis: All Steps Explained with Diagrams, Enzymes, Products and Energy Yield

February 6, 2019 Dhurba Giri 3

Glycolysis is the major pathway for the utilization of glucose in the body. Glycolysis is defined as the sequence of reactions for the breakdown of Glucose (6-carbon molecule) to two molecules of pyruvic acid (3-carbon […]

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