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convert-g-to-rpm
BIOCHEMISTRY

How to Convert ‘g’ to RPM and Vice Versa in a Centrifuge?

September 11, 2021 Dhurba Giri 1

Centrifuagtion is a technique that helps to separate mixtures by applying centrifugal force. A centrifuge machine works by using a principle of sedimentation. Under the influence of gravitational force, substances separate according to their density. […]

Diacetyl-monoxime-DAM-urea
BIOCHEMISTRY

Diacetyl Monoxime (DAM) Method for Estimation of Urea

March 28, 2021 Dhurba Giri 0

Urea is a waste product formed in liver following the breakdown of proteins. It passes into the blood, is filtered out of the kidneys and excreted in urine. Thus determination of blood urea is the […]

O-toluidine-method-for-glucose
BIOCHEMISTRY

O-Toluidine Method for Estimation of Blood Glucose

March 27, 2021 Dhurba Giri 0

The O-toluidine method is an older method of blood glucose estimation. This method is no longer used today because O-toluidine is believed to be a carcinogen and is replaced by enzymatic methods. This method is […]

Sulphosalicylic-Acid-Test
BIOCHEMISTRY

Sulphosalicylic Acid Test for Proteinuria: Principle and Procedure

March 21, 2021 Dhurba Giri 0

Most plasma proteins are too large to pass through the glomeruli of the kidney. The small amount of protein which does filter through is normally reabsorbed back into the blood by the kidney tubules. Only […]

Heat-and-acetic-acid-test
BIOCHEMISTRY

Heat and Acetic Acid Test for Proteinuria: Principle and Procedure

March 13, 2021 Dhurba Giri 0

Most plasma proteins are too large to pass through the glomeruli of the kidney. The small amount of protein which does filter through is normally reabsorbed back into the blood by the kidney tubules. Only […]

Arneth-Count
HEMATOLOGY

Arneth Count for Neutrophils: Principle, Procedure and Clinical Significance

January 2, 2021 Dhurba Giri 0

The Arneth count is the determination of the percentage distribution of different types of neutrophils on the basis of their nuclear lobes. It is not commonly used in modern medicine. Joseph Arneth classified neutrophils into […]

Gerhardts-Test
BIOCHEMISTRY

Gerhardt’s Test For Ketone Bodies: Principle, Procedure and Interpretation

December 12, 2020 Dhurba Giri 1

The term ketone bodies refers to three intermediate products of fat metabolism, namely acetone (2%), acetoacetic acid (20%) and beta-hydroxybutyrate (78%). Under normal conditions, metabolized fats are completely broken down to water and carbon dioxide, […]

laboratory-diagnosis-scrub-typhus
BACTERIOLOGY

Laboratory Diagnosis of Scrub Typhus

October 21, 2020 Dhurba Giri 0

Scrub typhus is an acute, febrile, infectious disease that is caused by bacteria called Orientia tsutsugamushi. It is a zoonotic disease (an animal disease that can spread to humans) primarily affecting rodents, rabbits and marsupials. […]

COVID-19-RT-PCR
MICROBIOLOGY

How COVID-19 RT-PCR Test Works? Principle and Protocol

September 20, 2020 Dhurba Giri 0

The coronavirus disease 2019 (COVID-19), is an illness caused by the most recently discovered coronavirus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It is an enveloped, positive sense, single-stranded ribonucleic acid (RNA) virus with […]

Wright's-Stain
CYTOPATHOLOGY

Wright’s Stain : Preparation, Principle, Procedure and Results

July 25, 2019 Dhurba Giri 4

Wright’s stain is a type of Romanowsky stain, which is commonly used in hematology laboratory for the routine staining of peripheral blood smears. It is also used for staining bone marrow aspirates, urine samples and […]

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