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Home2018

Year: 2018

Iron-deficiency-anemia
BIOCHEMISTRY

Laboratory Diagnosis of Iron Deficiency Anemia (IDA)

December 6, 2018 Dhurba Giri 2

Anemia is the condition of decrease in number of circulating red blood cells (and hence hemoglobin) below a normal range for age and sex of the individual, resulting in decreased oxygen supply to tissues. Iron […]

Sudan-Black-B-Stain
CYTOPATHOLOGY

Sudan Black B Stain : Purpose, Principle, Procedure and Interpretation

November 18, 2018 Dhurba Giri 1

Sudan Black B (SBB) is a fat soluble dye which has very high affinity for neutral fats and lipids. SBB staining is useful for for the differentiation of Acute myeloid leukemia (AML) from Acute lymphoid […]

Coagulase-test
BACTERIOLOGY

Coagulase Test : Types, Principle, Procedure, Interpretation and Examples

November 7, 2018 Dhurba Giri 0

Coagulase is an enzyme that works in conjugation with normal plasma components to convert fibrinogen to finbrin and causes clotting of plasma. This enzyme is a good indicator of pathogenic potential of Staphylococcus aureus. As […]

Differences-between-e-histolytica-and-e-coli
MICROBIOLOGY

Differences Between Entamoeba histolytica and Entamoeba coli

November 6, 2018 Dhurba Giri 6

Genus Entamoeba includes a group of amoebic parasites. Among them Entamoeba histolytica is an important human pathogen causing amoebic dysentery as well as hepatic amoebiasis. Entamoeba coli is a commensal of large intestine, but doesn’t […]

Myeloperoxidase-stain
CYTOPATHOLOGY

Myeloperoxidase (MPO) Stain : Purpose, Principle, Procedure and Interpretation

November 6, 2018 Dhurba Giri 5

Myeloperoxidase is a lysosomal enzyme present in the primary azurophilic granules of neutrophils, eosinophils and to a certain extent, monocytes. Lymphocytes do not contain myeloperoxidase. Purpose of Myeloperoxidase stain Myeloperoxidase (MPO) stain is useful for […]

Reticulocyte-Count
HEMATOLOGY

Reticulocyte Count : Principle, Procedure, Calculations and Clinical Significances

November 6, 2018 Dhurba Giri 7

Reticulocytes are immature red blood cells (RBCs) with considerable quantities of ribosomal and mitochondrial RNA. Normally, there are a small number of reticulocytes in the peripheral blood which remain for 24-48 hours during maturation. Reticulocyte […]

Hematoxylin and Eosin Staining
HISTOPATHOLOGY

Hematoxylin and Eosin (H&E) Staining : Principle, Procedure and Interpretation

November 5, 2018 Dhurba Giri 3

Hematoxylin and Eosin (H&E) staining is the most widely used staining technique in histopathology. As its name suggests, H&E stain makes use of a combination of two dyes, namely hematoxylin and eosin. This combination deferentially […]

Rothera's test for ketone bodies
BIOCHEMISTRY

Rothera’s Test for Ketone Bodies: Principle, Procedure and Clinical Significances

November 5, 2018 Dhurba Giri 5

The term ketone bodies refers to three intermediate products of fat metabolism, namely acetone (2%), acetoacetic acid (20%) and beta-hydroxybutyrate (78%). Under normal conditions, metabolized fats are completely broken down to water and carbon dioxide, […]

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