MCQs on Covid-19 and It’s Laboratory Diagnosis September 17, 2020 Dhurba Giri 0 Welcome to MCQs on Covid-19 and It's Laboratory Diagnosis. October 24, 2020 General Instructions for Participants: 1. Fill up your Name and email address before proceeding. No email verification is required. 2. This quiz contains 15 single answer multiple choice questions about Covid-19. Answer the questions by checking the corresponding checkbox infront of your answer. Only one answer is correct per question. 3. Once your answer is selected, it can't be changed. 4. Your score, highest score, your answers and correct answers will be displayed after you complete the quiz and submit your answers. Your Name* Email (optional) 1. The new strain of corona virus responsible for ongoing pandemic of coronavirus disease is: Covid-19 SARS-CoV SARS-CoV-2 MERS-CoV2. Covid-19 was declared as a pandemic by WHO on: 12 January 2020 11 March 2020 8 February 2020 22 March 20203. SARS-CoV-2 is classified within the genus: Alphacoronavirus Betacoronavirus Gammacoronavirus Deltacoronavirus4. Genetic Material of SARS-CoV-2 is composed of: Positive sense single stranded RNA (+ss RNA) Negative sense single stranded RNA (-ss RNA) Double stranded RNA (ds RNA) Double stranded DNA (ds DNA)5. Each virus attaches to a specific protein on the surface of the host cells. Which of the following is known to be a cell receptor for SARS-CoV-2? N-acetyl neuraminic acid Aminopeptidase N Intercellular adhesion molecule (ICAM-1) Angiotensin-converting enzyme 2 (ACE 2)6. What is the protein on the surface of the SARS-CoV-2 that binds to the target cells: M protein Capsid protein RNA dependent RNA polymerase (RdRP) Spike protein (S protein)7. Which of the following statements about ideal protocol for storage and transport of the samples in transport tubes is true? Specimens should be kept at room temperature (22°C) for storage and shipped within 72 hours Specimen should immediately be frozen and shipped frozen within 72 hours Specimen should be stored at 2-8°C and shipped on an ice pack within 72 hours Specimen should be stored and shipped with at room temperature8. Standard confirmation of acute SARS-CoV-2 infection is based on: Nucleic Acid Amplification Tests (NAATs) Serological Tests IgG and IgM Antibody Detection Viral Isolation9. A test for SARS-CoV-2, RT-PCR stands for: Real Time Polymerase Chain Reaction Reverse Transcription Polymerase Chain Reaction Rapid Technology Polymerase Chain Reaction Recombinant Technology Polymerase Chain Reaction10. The PCR technique was developed by: Kary Mullis Kohler Milstein Altman11. Which is the correct sequence order of RT-PCR? Annealing, Denaturation, Extension, Detection Denaturation, Extension, Annealing, Detection Denaturation, Annealing, Extension, Detection Annealing, Extension, Detection, Denaturation12. Nucleotide triphosphates (dNTPs) are added to the growing DNA strand during the _______________ phase. Denaturation Annealing Extension Extraction13. In RT-PCR, RNA is copied into complementary DNA (cDNA) by: Taq DNA Polymerase RNA Polymerase RNA dependent RNA polymerase (RdRP) Reverse Transcriptase II14. The viral genes targeted for Nucleic Acid Amplification Testing (NAAT) so far are: ORF open reading frame RdRp RNA-dependent RNA polymerase E gene All of the above15. Which is the Correct sequence of doffing of PPE? Gloves, Face shield, Gown, Mask Face shield, Gloves, Gown, Mask Gown, Face shield, Gloves, Mask Gown, Gloves, Face shield, MaskTime is Up!